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Advanced Cell Biology Research Article Assignment Not accepted late Associated Reading Golgi maturation visualized in living yeast Losev E Reinke CA Jellen J Strongin DE Bevis BJ Glick BS Nature 2006 Jun 22 441 7096 1002 6 Epub 2006 May 14 Paper and supplements are available more conveniently on LATTE Note Questions 1 6 are worth 1 point and will be graded individually on good faith effort This means that you do not need to have the correct answer to get full credit but you do need to demonstrate that you carefully read the paper and tried to answer the question correctly Any of these questions could appear on any of the tests If they do they will be graded for correctness Question 7 is optional and will not be graded How to submit This assignment must be submitted online via Latte as a pdf or text document 1 What is the big question of this paper Not what is this paper about but what problem is this entire field trying to solve What is the nature of the early cis medial and late trans TGN cisternae and how do they mature 2 Summarize the background in five sentences or less Bullet points are ok Determine florescence of early Golgi proteins tagged with GFP and late Golgi protein with DsRed using S cerevisiae The Golgi Dynamics were examined using 4D microscopy using markers Sec7 for late cisternae and VRG4 for early cisternae
