Essay Examples on Fitchburg State University

Plant material and Sterilization Shoot tips was selected and excised from peach baladai CV plant grown in Taif governorate The explants were used of peach was used as a plant materials The explants were disinfested for 1 min in 70 ethanol followed by 20 or 30 of Clorox solution 5 25 active ingredient sodium hypochlorite containing also 1500 ppm citric acid and 1500 ppm ascorbic acid for 20 or 30 minutes plus 0 01 Tween 20 and then rinsed three times with sterile distilled water Pooler and Scorza 1995 Growth chamber conditions were set to 24 ºC and a photoperiod of 16 hours at a light intensity of 2000 lx provided by cool white fluorescent tubes Media preparation Murashige and Skoog MS m supplemented with 3 sucrose w v and different concentrations of BAP 0 0 0 5 1 0 1 5 and 2 mg l were used for shoot initiation shoot multiplication and shoot elongation stages while different concentrations of IBA 0 0 0 1 1 0 and 2 mg l and 0 1 mg l NAA were used for root formation The pH of all media was adjusted to 5 7 using 1 0 N potassium hydroxide KOH and 1 0 N hydrochloric acid HCl before adding 0 8 w v phytoagar Media were autoclaved at 121 C for 20 min and 1 5 k cm2 pressure In vitro culture Meristems tip of 1 0 5 mm in length were taken under the stereoscope and cultured individually within glass test tubes and Shoot tips explants were cultured for three to four weeks on MS medium with various concentrations of BAP 0 0 0 5 1 0 1 5 and 2 mg l and 3 w v sucrose for shoot initiation multiplication and elongation Induced shoots were subcultured on the same media for another three to four weeks Rooting stage elongated shoots 3 to 4 cm length were transferred on MS medium with different concentrations of IBA 0 0 0 1 1 0 and 2 mg l in combination with 0 1 mg l of NAA for a further four weeks MS medium without growth regulators was used as a control for all the in vitro cultures