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Plants of 25 genotypes lines representing native as well as foreign Plants

2 Materials and methods The present field investigation were carried out during late kharif of 2013 and 2014 Instructional Farm Plant materials Plants of 25 genotypes lines representing native as well as foreign plants collected from different parts of India were maintained and considered for the present study Table 1 Newly emerged leaf samples of the cultivars were used for DNA extraction 2 2 Morphological analysis Seven morphometric characters were evaluated plant specimens from 25 genotypes lines Data on morphological characters were standardized using the YBAR option of the Stand program from the NTSYS pc 2 1 software Rohlf 2004 Duplicate measurements for each specimen were averaged and were used to design a data matrix of pairwise similarities between genotypes lines The simple matching coefficient SMC was used to measure the similarity as it was the coefficient with the best results following a cophenetic test Principal component analysis PCA was also used for non hierarchical relationships among the genotypes Eigenvalues and eigenvectors were calculated by the Eigen program using a correlation matrix as input calculated using standardized morphological data and a 2 D and 3 D plot was used to generate the two dimensional PCA plot from NTSYS pc 2 1 Rohlf 2004 2 3 Genomic DNA extraction and quantification

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