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Dermatophytosis can be transmitted from animal to animal and from human to animals Zoophilic fungi prefer animals as hosts but often cause acute inflammatory reactions when they invade humans Trichophyton verrucosum is the most common etiologic agent of cattle Trichophyton mentagrophytes Trichophyton rubrum Trichophyton simii and Microsporum gypseum are also bovine caused dermatophytosis The present study was designed to conduct laboratory diagnosis cutaneous fungal infections among cattle showing typical diagnostic features of superficial and cutaneous mycoses and to evaluate the in vitro inhibitory effect of zinc oxide nanoparticles ZnO on the growth of dermatophyte isolates In the present study ZnO nanoparticles ZnONPs were prepared using Pechini method In this technique stoichiometric amounts of Zn NO3 6H2O were weighed and well mixed with an equimolar solution of citric acid ethylene glycol This mixture was then transferred on a magnetic stirrer with a hot plate until drying After that an auto ignition takes places resulting in a fluffy white yellowish powder This powder was collected and then heated at 500 oC in Lenton Furnace UAF 16 5 The X ray powder diffraction XRD was carried out using a Proker D8 advance X ray diffractometer with CuK α radiation λ 1 5418 Å for the samples in the range of 20 80 The shape and morphology of the nanoparticles were analyzed using a High Resolution transmission electron microscope HRTEM model JEOl 2100 A total of fifty samples of skin scraping and hair were collected from the site of lesion after cleaning by cotton soaked in 70 alcohol from infected buffaloes and cows which have clinical manifestations of dermatophytosis
