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This experiment will investigate the effect of temperature on the rate of reaction of the enzyme catalyse Biological catalyst called enzymes increase the rate of reaction without themselves being chemically changed Enzymes are globular proteins folded into a complex 3 dimensional shape that contains a special surface area called the active site where a specific substrate binds structurally and chemically Enzymes speed up reactions as they lower the activation energy In this experiment the enzyme used is catalase which is found in both plant cells and animal cells The catalase I will use is yeast which has 37oC optimal temperature similar to the natural human temperature The substrate that will be catalyses is hydrogen peroxide H2O2 which is a poisonous waste product in the human body Therefore the catalase enzyme will specifically catalyse the hydrolysis reaction of H2O2 into safe non poisonous products of water and oxygen gas The reaction is 2H2O2 2H2O O2 g This experiment will specifically investigate the effect of temperature on the rate of reaction which can be calculated by measuring the speed of production formation In this experiment the production of O2 gas will determine the rate of reaction
