Essay Examples on University of New Haven

Plasmid DNA is a circular form of self-replicating

Plasmid DNA is a circular form of self-replicating DNA that scientists can manipulate to carry and clone other pieces of DNA Thieman Palladino 2009 strands are found in the cytoplasm of a bacterium that scientists can maneuver to conduct and clone other parts of DNA Plasmids is found in cells which the cells are then used as vectors because of their outstanding capability to transport genes that allow bacteria to grow in contrary circumstances Plasmids wouldn't be able to take in and break down without the help of restriction enzymes The goal for the enzymes is to cut the DNA at a specified sequence of nucleotides The goal here is to clear any DNA molecules that was not needed at the time of growth To determine the size fragments from how the restriction enzymes cut a tool called the Gel Electrophoresis was used Gel electrophoresis is one of the most accepted ways to figure out the DNA sequences that researchers have from DNA This skill will take assistance of the negative charge on DNA molecules by applying the electrical field which will implement the energy to abstract the DNA molecules proportioned to the size The goal of this experiment is to figure out the unknown plasmid that the instructor has given to help in the discovery of the unknown plasmid and there were three to figure out from pAMP pKAN or pBLU

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