Essay Example on Allergens are the substances that cause allergic Reactions

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Introduction Allergens are the substances that cause allergic reactions such as certain foods dust plant pollen or medicines An allergen is a type of antigen that produces an abnormally vigorous immune response in which the immune system fights off against perceived threat that would otherwise be harmless to the body In attempt to protect the body the immune system produces IgE antibodies mediated response to that allergen Those antibodies then cause certain cells in the body to release chemicals into the bloodstream such as histamine The histamine then acts on a person's eyes nose throat lungs skin or gastrointestinal tract and causes the symptoms of the allergic reaction Several factors affect allergens In this study some studies were conducted on herd to as allergen Farrokhi S Mousavi T Arshi S Varasteh A Rezaei N Salekmoghadam A Co Administration of Chenopodium Album Allergens and CpG Oligodeoxynucleotides Effects on Peripheral Blood Mononuclear Cells of Patients with Allergic Rhinitis Treated with Intranasal Corticosteroids and Antihistamines Iran J Allergy Asthma Immunol 2011 10 2 101 110 Chenopodium album is a fast growing weedy annual plant in the genus Chenopodium and its pollen act as allergen This study imbibe the preparation of pollen extract purification of antigen immunization production of antisera and the testing of antisera against allergy Amjad L Comparative Study of Pollen Extracts Allergenicity of Chenopodium album L and Chenopodium Botrys L an in vivo 



Study International conference on bioscience biochemistry and bioinformatics 2011 IPCBEE vol 5 2011 2011 IACSIT Press Singapore 338 341 Villalba M Barderas R Mas S Colás C Batanero E Rodríguez R Amaranthaceae Pollens Review of an Emerging Allergy in the Mediterranean Area J Investig Allergol Clin Immunol 2014 24 6 371 381 2 Material and methods 2 1 Mateiral 2 1 1 Pollen The Pollens of Chenopodium album CAP was collected from catkins of the plants in month of May from Roorkee The catkins plucked and dried at room temperature on a clean paper for two weeks at 40 ºC in shade to dry and pollens were separated by sieving the material through sieves of gradually reducing pore size and finally passed through muslin cloth with mild shaking The pollens were stored in closed glass containers at room temperature Similarly the pollen of C murale CMP and C virgatum CVP were collected Babu BNH Venkatesh YP Clinico Immunological Analysis of Eggplant Solanum melongena Allergy Indicates Preponderance of Allergens in the Peel World Allergy Organ J 2009 2 9 192 200 a b c Figure Photomicrograph of pollen grain at 1200X magnification a Chenopodium album b Chenopodium murale c Chenopodium virgatum 2 1 2 Animals White albino rabbits rabbits weighing 1 5 to 2 5 kg and albino guinea pigs weighing about 300 400 gm were used throughout the experimental work The animals were housed individually in polypropylene cages and maintained at 24 2 C under a 12 h light dark cycle and were fed ad libitum with standard pellet diet and had free access to water The animal experimental protocol was approved by the Institutional Animal Ethical Committee No India Visht S Kulkarni

GT Effect of some cations as counter ions on ulcer healing activity of glycyrrhetinic acid on male albino rats Int J Chem Sci 2015 13 3 1511 1521 Visht S Kulkarni GT Glycyrrhetinic acid ammonium loaded microspheres using Colocasia esculenta and Bombax ceiba mucilages In vitro and in vivo characterization 2016 Curr Drug Ther 11 101 114 2 2 Methodology 2 2 1 Preparation of antigenic extracts Crude extract of CAP All the steps were carried out at 4 C unless stated specifically Freshly obtained pollen grains were first defatted by repeated extraction with solvent ether until the colour of the grains stopped coming in the ether extract After defatting the pollen grains were dried in desiccators under suction The defatted and dried pollens were suspended in phosphate buffer saline pH 7 3 in the proportion of 1 10 w v containing 140 mM NaCl phosphate buffered saline PBS 2 mM phenyl methane sulfonyl fluoride PMSF 100 mM L ascorbic acid to prevent enzymic browning reaction and centrifuged at 5300 g for 20 min The suspension was vigorously agitated in a conical flask with glass beads for 24 h on an Emenvee Rotary Shaker After 24 h suspension was centrifuged at 3 000g for 20 minutes the supernatant was treated with 3 polyvinyl polypyrrolidone PVPP with continuous stirring for 30 min to remove interfering phenols centrifuged at 10000 g for 15 min and filtered through Whatman No 1 filter 11 µm pore size This was designated as stock solution of CAP allergen Similarly the crude extract of C murale CMP and C virgatum CVP were prepared Cooper HM Paterson Y Production of antibodies Section II Current Protocols in Immunology Copyright 1995 by John Wiley Sons Inc 1995 2 4 1 2 4 9


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