Essay Example on Endosulfan is highly lipophilic persistent organic insecticide

The biological alterations observed in an animal under chemical stress can be correlated with the structural and functional changes of cellular proteins Proteins hold the unique position in the modulators of cells because of the proteinaceuos nature of all the enzymes which mediate at various metabolic pathways Lehninger 2008 Harper 2006 Stress protein has been proposed as sensitive indicator of lethal exposure to contaminants in the environment When the cells are challenged with certain environmental stimulus such as temperature and toxic chemiacals cause the induction of stress protein genes the product of which function in new protein folding and in long effect it cause phenotypic change Craig et al 1989 Cruz Rodrique 2002 several stress protein have been shown to be induced by wide variety of stressors like hyperthermia heavy metal etc stress proteins are placed in to groups of families based on their relative in number and DNA sequence like high molecular weight stress protein stress protein 70 chaperones 60 stress protein 42 and low molecular weight stress proteins This chapter aims to determine median effective concentration EC50 of endosulfan alpha endosulfan and beta endosulfan to determine how much chemicals are needed to kill half of a group of test organisms in a given time And also evaluate the dose dependent stress inducing potential of endosulfan and its isomers on the life cycle of exposed drosophila by examining the emergence pattern hatchability and pupation Along with this the study of protein profile changes with differentially expressed proteins in D melanogaster treated with different doses of endosulfan alpha and beta endosulfan