Essay Example on Investigation of in vitro cytotoxicity and Antibacterial Properties









Investigation of in vitro cytotoxicity and antibacterial properties of Corchorus hirsutus phytoremediation nanosilver Dongamanti Ashok Sandupatla Raju Rama Koyati Green and Medicinal Chemistry Laboratory Department of Chemistry Osmania University Hyderabad 500007 Telangana India Forensic Science Unit Department of Chemistry Osmania University Hyderabad 500007 Telangana India Department of Biochemistry Osmania University Hyderabad 500007 Telangana India email address ashokdou gmail com Tel 91 9391024769 Abstract The elected work reports for the first time successfully synthesized silver nanoparticles from the aqueous leaf extract of the Corchorus hirsutus plant As the phytochemicals presented in the leaf extract were creditworthy for the reduction of silver ions as the stable nanosilver particles as well as they act as the capping agents which were environed on the surface of the particles which increases the stability of the particles The phytosynthesized nanosilver were then characterized by using several instrumental techniques The establishment of nanosilver was overseen by UV Vis spectroscopy The precise peak detected at 403 nm of surface plasmon resonance has affirmed the colloidal nanosilver The peaks of fourier transform infrared spectroscopy had found out the phytochemicals responsible for the reduction of nanosilver The crystalline structure and diameter of the phytosynthesized silver nanoparticles were found out by using x ray diffraction studies The average diameter of particles found to be 29 nm using Debye Scherrer formula 

The scanning electron microscopy images rendering the aggregated semi-spherical to spherical silver nanocrystals synthesized from Corchorus hirsutus The elemental signals of energy dispersive x-ray spectrum substantiate the presence of silver element as silver weight is 86 84 and atomic is 68 45 The atomic force microscope was used to examine the size shape and the topographical structure of the synthesized nanosilver It furnishes the 3D images of particles on the surface of the sample Then the antibacterial activity of synthesized nanosilver has been successfully tested by well diffusion method against four harmful bacteria Staphylococcus aureus Klebsiella pneumoniae Pseudomonas putida and Bacillus subtilis as evincing high activity The Corchorus hirsutus mediated nanosilver exerts their cytotoxic effect on MCF7 cell lines of breast adenocarcinoma cells and HeLa cell lines of cervical cancer cells Keywords Corchorus hirsutus nanosilver atomic force microscope anticancer antimicrobial properties 1 Introduction

Present age Nanotechnology is the trending research area Around the globe researchers from all fields of science and technology are doing research in nanoscience because of their wide range of applications The vast range of novel applications was accomplished in the current age of metallic nanoparticles that s why broader research has been continuing in this field and more has to be done The epoch of nanotechnology and nano-related discovering in the present has accelerated towards new technological developments and applications which imparts the interest in the industrial and scientific community The nanoparticles are dealing with the sizes below 100 nm range as the smallest particles the activity was good due increasing the surface 1 area when compared to the larger in size and also the properties vary with the shape surface structure and distribution

These all parameters are dependent on discovering novel properties which have the consequence to be used in various novel applications 2 So the nanotechnology is the coming forth field because it is everywhere in all areas of science and technology such as physics chemistry engineering electronics information technology biochemistry biotechnology biomedical engineering medicine materials science forensic science 3 textile and food technology 4 and agriculture 5 and many more areas In most of the cases the silver nanoparticles constituted are highly unstable and necessitate the addition of a separate capping agent which renders stability It has been widely reported that synthesis of nanosilver using plant extracts often leads to highly controlled size and morphology Moreover such pathways are cost-effective eco friendly 6 do not use toxic chemicals high pressure and temperatures furthermore the written reports unveiled that the nanosilver synthesized from sustainable or green chemistry methods possess potent antibacterial properties 7 10 

The establishment of plant-mediated metallic nanoparticles was a utilitarian area of research because the plants owning the phytochemicals and their active compounds which were used to cure lots of diseases as they medically important A wide range of literature available in the synthesis of nanoparticles from phytoremediation green chemistry methods 11 18 So the involvement of these phytochemicals from medicinal plants environed by nanoparticles and the combination of these active compounds with metallic nanoparticles evinced a wide range of innovative and improved applications The picked out plant Corchorus hirsutus was used for the first time for the synthesis of nanosilver Its common names were Wooly Booger and Jack Switch belongs to the family Tiliaceae The Corchorus hirsutus grows as a small to medium shrub up to 2m in height The leaves are arranged alternately to 8 cm in length and 3 cm in width ovate to oblong with a serrate leaf margin and a rounded leaf apex 

The Corchorus hirsutus leaves edible after cooking and made into tea Jack switch furnishes cover for wildlife and helps protect the soil The leaves were used to treat colds and flu Branches were formerly used to make brooms The current investigation reported towards the synthesis of surfactant-free metallic silver nanoparticles through the eco-friendly biological root using a medicinal plant Carchorus hirsutus Therefore the objectives of the present study are to the synthesis of silver nanoparticles using Carchorus hirsutus aqueous leaves extract as reducing as well as capping agent to characterize the synthesized nanosilver to evaluate the antibacterial property against four tested harmful bacteria and study of in vitro cytotoxic properties against MCF7 HeLa cell lines

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