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343They are aerobic and chemoorganotrophic Zoogloea ramigera 1 16 M was found to Zoogloea ramigera has been found to contain two stereospecific acetoacetyl CoA reductases 1 NADP linked with D β fi hydroxybutyryl CoA specific 2 NAD linked and L isomer specific Saito Fukui Ikeda Tanaka Tomita 2000 The NADP linked enzyme has a pH optimum for the reduction of acetoacetyl CoA at PH of 8 1 however for the oxidation of hydroxybutyryl CoA optimum PH is non definite Zoogloea is not choosy nutritionally thus a variety of organic carbon sources in a simple defined media may be used to culture it Gerardi 2007 Poly β hydroxybutyrate PHB is produced whenever both a carbon source is highly present as well as B ketothiolase PHB synthesis and intracellular degradation is partially controlled by NADPH and availability through glucose 6 phosphate dehydrogenase enzyme with optimum operating PH of 8 5 The species is also known to use Mn oxidation metabolism because the matrix made by Zoogloea takes in and concentrated metal in water sources Range for pH temperature salt concentration The preferred temperature for growth is 28 37 degrees C Zooglea ramigera flourish in an environment with a PH of 7 0 7 5 The formation of flocs and films in liquid media at late stages of growth are distinguished by treelike or fingerlike morphology Colonies on C4 agar at 3 4
days reach 1mm in diameter are circular raised translucent with opaque centers of gray or white Mature colonies are tenacious and cohesive and can be lifted from agar surface intact with a needle Control of the environment for Zooglea ramigera can be achieved via PH and temperature Similar to its metabolic activities the preferred temperature for growth is 28 37 degrees Zooglea ramigera flourish in an environment with a PH of 7 0 7 5 because a decrease in PH of the activated sludge process favors the proliferation of filamentous fungi and disfavors the growth of bacteria Angelbeck Kirsch 1969 Nutrient type addition and control is usually recommended Zoogleal bulking occurs when the bacteria takes too long to access nutrients in an environment with high BOD loading or nutrient slug feeding rate is not consistent or when the nutrient is not sufficient According to Richard Ph 2003 conditions that favor Zooglea or other microbes responsible for viscous bulking are High levels of soluble organics in the influent and imbalance in macronutrient C N P favoring organisms that an uptake scarce nutrients such as Zooglea ramigera Possible interactions with other micro organisms Transfer of carbon and energy through a food web or food chain interrelates organisms in an ecosystem Each organism has an effect on other organisms symbiotic or predator prey relationships Free swimming ciliated protozoa multiply in number in the presence of large numbers of dispersed Zooglea ramigera bacterial cells During floc formation the number of dispersed Zooglea ramigera bacterial cells decreases and as a result to decrease in the number of free swimming ciliated protozoa