Separation of most blood cells is difficult if not impossible to achieve because they have similar properties and or densities What procedure is used to separate T cells of the immune system from the many other different types of white blood cells or spleen cells What feature of the T cell facilitates the isolation protocol Describe how this procedure is performed T cells carry proteins CD3 and Thy 1 2 on their surface When antibodies that are linked to fluorescent dye and specific for these proteins are incubated with a mix of cells only the T cells will illuminate because they express the specific proteins and are recognized by the antibodies that carry dye A fluorescence activated cell sorter which is based on flow cytometry can separate cells that emit different levels of fluorescence